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Assay Detail

CHEMBL4255804

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Staphylococcus aureus Newman Hi6-tagged CrtN expressed in Escherichia coli BL21(DE3))/pET28a::CrtN assessed as reduction in staphyloxanthin levels using diapophytoene as substrate after overnight incubation
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus subsp. aureus str. Newman
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) (CHEMBL3813586)
Type SINGLE PROTEIN
Organism Staphylococcus aureus (strain Newman)

Publication

Novel Staphyloxanthin Inhibitors with Improved Potency against Multidrug Resistant Staphylococcus aureus.
Ni S, Li B, Chen F, Wei H, Mao F, Liu Y, Xu Y, Qiu X, Li X, Liu W, Hu L, Ling D, Wang M, Zheng X, Zhu J, Lan L, Li J.
ACS Med Chem Lett (2018) 9 : 233 -237
PubMed 29541366 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.53 6.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4284707 1 6.59
CHEMBL4288408 1 6.57
CHEMBL3827961 1 6.50
CHEMBL4278050 1 6.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4284707 IC50 = 258.4 nM 6.59
CHEMBL4288408 IC50 = 270.4 nM 6.57
CHEMBL3827961 IC50 = 320.0 nM 6.50
CHEMBL4278050 IC50 = 340.7 nM 6.47