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Assay Detail

CHEMBL4256086

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Staphylococcus aureus MraY expressed in Escherichia coli membrane using Park's nucleotide as substrate pretreated for 30 mins followed by substrate addition measured up to 2 mins by fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Phospho-N-acetylmuramoyl-pentapeptide-transferase (CHEMBL1770035)
Type SINGLE PROTEIN
Organism Staphylococcus aureus

Publication

Antibacterial Muraymycins from Mutant Strains of Streptomyces sp. NRRL 30471.
Cui Z, Wang X, Koppermann S, Thorson JS, Ducho C, Van Lanen SG.
J Nat Prod (2018) 81 : 942 -948
PubMed 29553733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 10.50 10.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4286766 1 10.80
CHEMBL4279378 1 10.66
CHEMBL4284923 1 10.03
CHEMBL4283345 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4286766 IC50 = 0.0157 nM 10.80
CHEMBL4279378 IC50 = 0.0221 nM 10.66
CHEMBL4284923 IC50 = 0.093 nM 10.03
CHEMBL4283345 IC50 = 0.004 nM -