Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4256769

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human sEH in human HepG2 cells using (+/-)14(15)-EET-d11 as substrate assessed as formation of (+/-)14(15)-DHET-d11 preincubated for 15 mins followed by substrate addition and measured after 10 mins by LC-MS/MS analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bifunctional epoxide hydrolase 2 (CHEMBL2409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Boosting Anti-Inflammatory Potency of Zafirlukast by Designed Polypharmacology.
Schierle S, Flauaus C, Heitel P, Willems S, Schmidt J, Kaiser A, Weizel L, Goebel T, Kahnt AS, Geisslinger G, Steinhilber D, Wurglics M, Rovati GE, Schmidtko A, Proschak E, Merk D.
J Med Chem (2018) 61 : 5758 -5764
PubMed 29878767 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.85 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4279086 1 7.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4279086 IC50 = 14.0 nM 7.85