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Assay Detail

CHEMBL4256812

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to N-terminal His-tagged human recombinant XIAP BIR3 (253 to 347 residues) expressed in Escherichia coli BL21(DE3) Gold by isothermal titration calorimetric analysis
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase XIAP (CHEMBL4198)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design of Potent pan-IAP and Lys-Covalent XIAP Selective Inhibitors Using a Thermodynamics Driven Approach.
Baggio C, Gambini L, Udompholkul P, Salem AF, Aronson A, Dona A, Troadec E, Pichiorri F, Pellecchia M.
J Med Chem (2018) 61 : 6350 -6363
PubMed 29940121 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 33 6.46 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4288451 1 7.16
CHEMBL4277802 1 7.09
CHEMBL4278294 1 7.09
CHEMBL4288478 1 7.07
CHEMBL2063869 GDC-0152 1.0 1 7.06
CHEMBL4276715 1 6.96
CHEMBL4282156 1 6.91
CHEMBL4287773 1 6.88
CHEMBL4283320 1 6.81
CHEMBL4295015 1 6.81
CHEMBL4288974 1 6.74
CHEMBL4284370 1 6.72
CHEMBL4278366 1 6.71
CHEMBL4291176 1 6.69
CHEMBL4292374 1 6.67
CHEMBL4281757 1 6.64
CHEMBL4286273 1 6.64
CHEMBL4284639 1 6.57
CHEMBL4294581 1 6.55
CHEMBL4291873 1 6.49
CHEMBL4291444 1 6.47
CHEMBL4286676 1 6.46
CHEMBL4279856 1 6.45
CHEMBL4280168 1 6.41
CHEMBL4285586 1 6.22
CHEMBL4287457 1 6.14
CHEMBL4288043 1 6.12
CHEMBL4283597 1 6.11
CHEMBL4290043 1 6.00
CHEMBL4281250 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4288451 Kd = 70.0 nM 7.16
CHEMBL4277802 Kd = 81.0 nM 7.09
CHEMBL4278294 Kd = 81.0 nM 7.09
CHEMBL4288478 Kd = 86.0 nM 7.07
CHEMBL2063869 GDC-0152 Kd = 88.0 nM 7.06
CHEMBL4276715 Kd = 110.0 nM 6.96
CHEMBL4282156 Kd = 122.0 nM 6.91
CHEMBL4287773 Kd = 133.0 nM 6.88
CHEMBL4283320 Kd = 155.0 nM 6.81
CHEMBL4295015 Kd = 155.0 nM 6.81
CHEMBL4288974 Kd = 182.0 nM 6.74
CHEMBL4284370 Kd = 190.0 nM 6.72
CHEMBL4278366 Kd = 194.0 nM 6.71
CHEMBL4291176 Kd = 206.0 nM 6.69
CHEMBL4292374 Kd = 213.0 nM 6.67
CHEMBL4286273 Kd = 230.0 nM 6.64
CHEMBL4281757 Kd = 227.0 nM 6.64
CHEMBL4284639 Kd = 269.0 nM 6.57
CHEMBL4294581 Kd = 280.0 nM 6.55
CHEMBL4291873 Kd = 321.0 nM 6.49
CHEMBL4291444 Kd = 337.0 nM 6.47
CHEMBL4286676 Kd = 346.0 nM 6.46
CHEMBL4279856 Kd = 354.0 nM 6.45
CHEMBL4280168 Kd = 386.0 nM 6.41
CHEMBL4285586 Kd = 602.0 nM 6.22
CHEMBL4287457 Kd = 719.0 nM 6.14
CHEMBL4288043 Kd = 753.0 nM 6.12
CHEMBL4283597 Kd = 783.0 nM 6.11
CHEMBL4290043 Kd = 1000.0 nM 6.00
CHEMBL4281250 Kd = 1100.0 nM 5.96
CHEMBL4276810 Kd = 12000.0 nM 4.92
CHEMBL4284657 Kd = 13000.0 nM 4.89
CHEMBL4280144 Kd = 17000.0 nM 4.77