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Assay Detail

CHEMBL4256813

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of AVPIAQKSEK-biotin from N-terminal His-tagged human recombinant XIAP BIR3 (253 to 347 residues) expressed in Escherichia coli BL21(DE3) Gold after 1 hr by DELFIA
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase XIAP (CHEMBL4198)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design of Potent pan-IAP and Lys-Covalent XIAP Selective Inhibitors Using a Thermodynamics Driven Approach.
Baggio C, Gambini L, Udompholkul P, Salem AF, Aronson A, Dona A, Troadec E, Pichiorri F, Pellecchia M.
J Med Chem (2018) 61 : 6350 -6363
PubMed 29940121 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.04 7.95

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4277819 1 7.95
CHEMBL4281278 1 7.78
CHEMBL4288451 1 7.63
CHEMBL4291176 1 7.46
CHEMBL4288478 1 7.43
CHEMBL4283320 1 7.42
CHEMBL4291896 1 7.33
CHEMBL2063869 GDC-0152 1.0 1 7.28
CHEMBL4276715 1 7.11
CHEMBL4277802 1 7.06
CHEMBL4280168 1 6.97
CHEMBL4288043 1 6.75
CHEMBL4283597 1 6.72
CHEMBL4276740 1 6.72
CHEMBL4281250 1 6.67
CHEMBL4291444 1 6.64
CHEMBL4291873 1 6.56
CHEMBL4284657 1 5.23
CHEMBL4280144 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4277819 IC50 = 11.3 nM 7.95
CHEMBL4281278 IC50 = 16.6 nM 7.78
CHEMBL4288451 IC50 = 23.2 nM 7.63
CHEMBL4291176 IC50 = 35.0 nM 7.46
CHEMBL4288478 IC50 = 37.1 nM 7.43
CHEMBL4283320 IC50 = 38.3 nM 7.42
CHEMBL4291896 IC50 = 47.3 nM 7.33
CHEMBL2063869 GDC-0152 IC50 = 52.7 nM 7.28
CHEMBL4276715 IC50 = 76.9 nM 7.11
CHEMBL4277802 IC50 = 86.8 nM 7.06
CHEMBL4280168 IC50 = 108.2 nM 6.97
CHEMBL4288043 IC50 = 175.9 nM 6.75
CHEMBL4283597 IC50 = 190.7 nM 6.72
CHEMBL4276740 IC50 = 189.4 nM 6.72
CHEMBL4281250 IC50 = 215.3 nM 6.67
CHEMBL4291444 IC50 = 229.4 nM 6.64
CHEMBL4291873 IC50 = 275.7 nM 6.56
CHEMBL4284657 IC50 = 5944.0 nM 5.23
CHEMBL4280144 IC50 > 10000.0 nM -