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Assay Detail

CHEMBL4256920

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of miR-21 RNA in human A498 cells assessed as potentiation of topotecan-induced cytotoxicity by measuring topotecan IC50 at 10 uM after 72 hrs by XTT assay (Rvb = 1 uM)
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 3 — Cell-line / Tissue
Curated By Intermediate

Target

microRNA 21 (CHEMBL1615322)
Type NUCLEIC-ACID
Organism Homo sapiens

Publication

Small Molecule Inhibition of MicroRNA miR-21 Rescues Chemosensitivity of Renal-Cell Carcinoma to Topotecan.
Naro Y, Ankenbruck N, Thomas M, Tivon Y, Connelly CM, Gardner L, Deiters A.
J Med Chem (2018) 61 : 5900 -5909
PubMed 29993250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.05 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4280638 1 7.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4280638 IC50 = 90.0 nM 7.05