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Assay Detail

CHEMBL4257192

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant CYP21 (unknown origin) expressed in human AD293 cells assessed as reduction in 11-deoxycortisol level preincubated for 60 mins followed by 17alpha-OH-progesterone substrate addition and measured after 45 mins by LC-MS method
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Steroid 21-hydroxylase (CHEMBL2759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel functionalized 5-(phenoxymethyl)-1,3-dioxane analogs exhibiting cytochrome p450 inhibition and their method of use
(2016)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.10 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4290805 1 7.17
CHEMBL4287608 1 6.96
CHEMBL4291856 1 6.89
CHEMBL4284316 1 6.77
CHEMBL4289930 1 6.68
CHEMBL4283588 1 6.58
CHEMBL4283318 1 6.56
CHEMBL4279058 1 6.41
CHEMBL4279441 1 6.38
CHEMBL4288383 1 6.37
CHEMBL4278076 1 6.24
CHEMBL4281307 1 6.10
CHEMBL4277054 1 6.10
CHEMBL4286761 1 6.02
CHEMBL4284620 1 5.96
CHEMBL4283034 1 5.83
CHEMBL4285420 1 5.82
CHEMBL4280259 1 5.60
CHEMBL4278033 1 5.55
CHEMBL4281673 1 5.32
CHEMBL4283221 1 5.00
CHEMBL4288652 1 5.00
CHEMBL4285744 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4290805 IC50 = 68.0 nM 7.17
CHEMBL4287608 IC50 = 110.0 nM 6.96
CHEMBL4291856 IC50 = 130.0 nM 6.89
CHEMBL4284316 IC50 = 170.0 nM 6.77
CHEMBL4289930 IC50 = 208.0 nM 6.68
CHEMBL4283588 IC50 = 262.0 nM 6.58
CHEMBL4283318 IC50 = 273.0 nM 6.56
CHEMBL4279058 IC50 = 391.0 nM 6.41
CHEMBL4279441 IC50 = 415.0 nM 6.38
CHEMBL4288383 IC50 = 426.0 nM 6.37
CHEMBL4278076 IC50 = 570.0 nM 6.24
CHEMBL4281307 IC50 = 800.0 nM 6.10
CHEMBL4277054 IC50 = 800.0 nM 6.10
CHEMBL4286761 IC50 = 960.0 nM 6.02
CHEMBL4284620 IC50 = 1100.0 nM 5.96
CHEMBL4283034 IC50 = 1480.0 nM 5.83
CHEMBL4285420 IC50 = 1520.0 nM 5.82
CHEMBL4280259 IC50 = 2500.0 nM 5.60
CHEMBL4278033 IC50 = 2800.0 nM 5.55
CHEMBL4281673 IC50 = 4760.0 nM 5.32
CHEMBL4283221 IC50 = 10000.0 nM 5.00
CHEMBL4288652 IC50 = 10000.0 nM 5.00
CHEMBL4285744 IC50 = 10000.0 nM 5.00