Assay Detail
Binding
CHEMBL4257308
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His6-Smt3-fusion tagged human Usp9x catalytic domain (1553 to 1960 residues) expressed in Escherichia coli pre- incubated for 30 mins before Ub-AMC substrate addition by fluorescence based assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Ubiquitin carboxyl-terminal hydrolase 9X (CHEMBL2406899) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Deubiquitinase inhibitors and methods for use of the same
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.40 | 5.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4279461 | — | — | 1 | 5.92 |
| CHEMBL4287357 | — | — | 1 | 5.80 |
| CHEMBL4278343 | — | — | 1 | 5.70 |
| CHEMBL4293176 | — | — | 1 | 5.34 |
| CHEMBL1923233 | — | — | 1 | 5.32 |
| CHEMBL4292817 | — | — | 1 | 5.29 |
| CHEMBL4283401 | — | — | 1 | 5.22 |
| CHEMBL4291403 | — | — | 1 | 5.21 |
| CHEMBL4277327 | — | — | 1 | 5.21 |
| CHEMBL4279846 | — | — | 1 | 4.97 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4279461 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL4287357 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL4278343 | — | IC50 | = | 1990.0 | nM | 5.70 |
| CHEMBL4293176 | — | IC50 | = | 4610.0 | nM | 5.34 |
| CHEMBL1923233 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL4292817 | — | IC50 | = | 5120.0 | nM | 5.29 |
| CHEMBL4283401 | — | IC50 | = | 5970.0 | nM | 5.22 |
| CHEMBL4291403 | — | IC50 | = | 6230.0 | nM | 5.21 |
| CHEMBL4277327 | — | IC50 | = | 6130.0 | nM | 5.21 |
| CHEMBL4279846 | — | IC50 | = | 10730.0 | nM | 4.97 |