Assay Detail
Binding
CHEMBL4258182
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Inhibition of Mycobacterium tuberculosis ICL1 expressed in Escherichia coli BL21(DE3) using DL-isocitrate as substrate incubated for 25 mins in presence of MgCl2 by 1H NMR method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mycobacterium tuberculosis |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
Isocitrate lyase 1 (CHEMBL4295587) ↗| Type | SINGLE PROTEIN |
| Organism | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Publication
Development of NMR and thermal shift assays for the evaluation of <i>Mycobacterium tuberculosis</i> isocitrate lyase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 4.71 | 4.83 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL451226 | — | — | 1 | 4.83 |
| CHEMBL177837 | BROMOPYRUVATE | — | 1 | 4.76 |
| CHEMBL359159 | ITACONATE | — | 1 | 4.53 |
| CHEMBL4293898 | — | — | 1 | - |
| CHEMBL1497581 | — | — | 1 | - |
| CHEMBL4226192 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL451226 | — | IC50 | = | 14700.0 | nM | 4.83 |
| CHEMBL177837 | BROMOPYRUVATE | IC50 | = | 17500.0 | nM | 4.76 |
| CHEMBL359159 | ITACONATE | IC50 | = | 29400.0 | nM | 4.53 |
| CHEMBL4293898 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL1497581 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL4226192 | — | IC50 | = | 250000.0 | nM | - |