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Assay Detail

CHEMBL4258267

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human FKBP12 expressed in Escherichia coli using succinyl-Ala-Phe-Pro-Phe-4-nitroanilide as substrate measured after 5 to 10 mins by spectrophotometric method
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peptidyl-prolyl cis-trans isomerase FKBP1A (CHEMBL1902)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of rapamycin-derived, next generation small molecules.
Guduru SKR, Arya P.
Medchemcomm (2018) 9 : 27 -43
PubMed 30108899 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 8.45 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL413 SIROLIMUS 4.0 1 9.22
CHEMBL4276944 1 9.00
CHEMBL4291053 1 9.00
CHEMBL4287849 1 9.00
CHEMBL4291011 1 9.00
CHEMBL4280369 1 9.00
CHEMBL4279943 1 8.82
CHEMBL4281041 1 8.52
CHEMBL4287588 1 8.46
CHEMBL216404 1 8.43
CHEMBL4283828 1 8.35
CHEMBL4279511 1 8.30
CHEMBL4283415 1 8.22
CHEMBL4291680 1 8.15
CHEMBL4280419 1 8.15
CHEMBL4295102 1 8.05
CHEMBL4291252 1 8.00
CHEMBL4290182 1 7.54
CHEMBL4284445 1 7.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL413 SIROLIMUS Ki = 0.6 nM 9.22
CHEMBL4276944 Ki = 1.0 nM 9.00
CHEMBL4291053 Ki = 1.0 nM 9.00
CHEMBL4287849 Ki = 1.0 nM 9.00
CHEMBL4291011 Ki = 1.0 nM 9.00
CHEMBL4280369 Ki = 1.0 nM 9.00
CHEMBL4279943 Ki = 1.5 nM 8.82
CHEMBL4281041 Ki = 3.0 nM 8.52
CHEMBL4287588 Ki = 3.5 nM 8.46
CHEMBL216404 Ki = 3.7 nM 8.43
CHEMBL4283828 Ki = 4.5 nM 8.35
CHEMBL4279511 Ki = 5.0 nM 8.30
CHEMBL4283415 Ki = 6.0 nM 8.22
CHEMBL4291680 Ki = 7.0 nM 8.15
CHEMBL4280419 Ki = 7.0 nM 8.15
CHEMBL4295102 Ki = 9.0 nM 8.05
CHEMBL4291252 Ki = 10.0 nM 8.00
CHEMBL4290182 Ki = 29.0 nM 7.54
CHEMBL4284445 Ki = 38.0 nM 7.42