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Assay Detail

CHEMBL4258458

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged recombinant human IDH1 R132H mutant expressed in Escherichia coli Rosetta (DE3) preincubated for 30 mins followed by NADPH/alpha-KG addition and measured after 1 hr
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of novel 5-hydroxy-2-methyl-4<i>H</i>-pyran-4-one derivatives as antiglioma agents.
Li YB, Hou W, Lin H, Sun PH, Lin J, Chen WM.
Medchemcomm (2018) 9 : 471 -476
PubMed 30108937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.41 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2180727 1 7.10
CHEMBL4286032 1 4.64
CHEMBL4278116 1 4.50
CHEMBL4280797 1 -
CHEMBL4280398 1 -
CHEMBL4291032 1 -
CHEMBL4282653 1 -
CHEMBL4293250 1 -
CHEMBL4294325 1 -
CHEMBL4286444 1 -
CHEMBL4279183 1 -
CHEMBL4289867 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2180727 IC50 = 80.0 nM 7.10
CHEMBL4286032 IC50 = 23100.0 nM 4.64
CHEMBL4278116 IC50 = 32000.0 nM 4.50
CHEMBL4280797 IC50 > 50000.0 nM -
CHEMBL4280398 IC50 > 50000.0 nM -
CHEMBL4291032 IC50 > 50000.0 nM -
CHEMBL4282653 IC50 > 50000.0 nM -
CHEMBL4293250 IC50 > 50000.0 nM -
CHEMBL4294325 IC50 > 50000.0 nM -
CHEMBL4286444 IC50 > 50000.0 nM -
CHEMBL4279183 IC50 > 50000.0 nM -
CHEMBL4289867 IC50 > 50000.0 nM -