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Assay Detail

CHEMBL4259681

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human MAO-A assessed as reduction in H2O2 production preincubated for 30 mins followed by p-tyramine substrate addition measured after 30 mins by amplex red reagent based fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Amine oxidase [flavin-containing] A (CHEMBL1951)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Curcumin-based pyrazoline analogues as selective inhibitors of human monoamine oxidase A.
Nath C, Badavath VN, Thakur A, Ucar G, Acevedo O, Mohd Siddique MU, Jayaprakash V.
Medchemcomm (2018) 9 : 1164 -1171
PubMed 30109004 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 5.54 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4289477 1 7.10
CHEMBL86304 MOCLOBEMIDE 4.0 1 6.99
CHEMBL972 SELEGILINE 4.0 1 5.44
CHEMBL4290556 1 4.71
CHEMBL4287129 1 4.58
CHEMBL4279206 1 4.40
CHEMBL279390 LAZABEMIDE 2.0 1 -
CHEMBL4282674 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4289477 Ki = 80.0 nM 7.10
CHEMBL86304 MOCLOBEMIDE Ki = 102.0 nM 6.99
CHEMBL972 SELEGILINE Ki = 3600.0 nM 5.44
CHEMBL4290556 Ki = 19550.0 nM 4.71
CHEMBL4287129 Ki = 26200.0 nM 4.58
CHEMBL4279206 Ki = 40000.0 nM 4.40
CHEMBL279390 LAZABEMIDE Ki = 7900000.0 nM -
CHEMBL4282674 Ki = 192840.0 nM -