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Assay Detail

CHEMBL4262016

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal FLAG-tagged full length recombinant human HDAC2 expressed in baculovirus infected Sf9 insect cells after 3 hrs by FDL assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 2 (CHEMBL1937)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Quantitative measurement of intracellular HDAC1/2 drug occupancy using a <i>trans</i>-cyclooctene largazole thiol probe.
Xu H, Roberts LR, Chou S, Pierce B, Narayanan A, Jones LH.
Medchemcomm (2017) 8 : 767 -770
PubMed 30108795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.30 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL356066 DACINOSTAT 2.0 1 8.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL356066 DACINOSTAT IC50 = 5.0 nM 8.30