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Assay Detail

CHEMBL4262865

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of phosphorylated FGFR3 (411 to 806 residues) (unknown origin) using 5-Fluo-Ahx-EEPLYWSFPAKKKCONH2 as substrate after 60 mins by microfluidic mobility shift assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 3 (CHEMBL2742)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Approaches to selective fibroblast growth factor receptor 4 inhibition through targeting the ATP-pocket middle-hinge region.
Fairhurst RA, Knoepfel T, Leblanc C, Buschmann N, Gaul C, Blank J, Galuba I, Trappe J, Zou C, Voshol J, Genick C, Brunet-Lefeuvre P, Bitsch F, Graus-Porta D, Furet P.
Medchemcomm (2017) 8 : 1604 -1613
PubMed 30108871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.08 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4284069 1 8.77
CHEMBL1852688 INFIGRATINIB 4.0 1 8.74
CHEMBL3348846 FEXAGRATINIB 2.0 1 8.28
CHEMBL509032 TAE-684 1 7.07
CHEMBL4068896 1 6.11
CHEMBL4279514 1 5.46
CHEMBL2403844 1 5.10
CHEMBL4277111 1 -
CHEMBL4294665 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4284069 IC50 = 1.7 nM 8.77
CHEMBL1852688 INFIGRATINIB IC50 = 1.8 nM 8.74
CHEMBL3348846 FEXAGRATINIB IC50 = 5.2 nM 8.28
CHEMBL509032 TAE-684 IC50 = 85.0 nM 7.07
CHEMBL4068896 IC50 = 780.0 nM 6.11
CHEMBL4279514 IC50 = 3500.0 nM 5.46
CHEMBL2403844 IC50 = 8000.0 nM 5.10
CHEMBL4277111 IC50 > 10000.0 nM -
CHEMBL4294665 IC50 > 10000.0 nM -