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Assay Detail

CHEMBL4263113

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at N-terminal FLAG-tagged human full-length GnRHR expressed in HEK293T cells assessed as inhibition of GnRH-induced calcium mobilization preincubated for 15 mins followed by agonist addition by FLIPR assay
26
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecule piperazinyl-benzimidazole antagonists of the gonadotropin-releasing hormone (GnRH) receptor.
Fjellaksel R, Boomgaren M, Sundset R, Haraldsen IH, Hansen JH, Riss PJ.
Medchemcomm (2017) 8 : 1965 -1969
PubMed 30108717 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.64 8.30
Ki 13 7.35 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL71917 2 10.00
CHEMBL515123 2 7.91
CHEMBL4283577 2 7.86
CHEMBL494803 2 7.72
CHEMBL4282476 2 7.42
CHEMBL4285058 2 6.98
CHEMBL4286937 2 6.80
CHEMBL4294163 2 6.73
CHEMBL4280124 2 6.59
CHEMBL4290761 2 6.40
CHEMBL4277116 2 6.39
CHEMBL4279003 2 -
CHEMBL4290349 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL71917 Ki = 0.1 nM 10.00
CHEMBL71917 IC50 = 5.012 nM 8.30
CHEMBL515123 Ki = 12.2 nM 7.91
CHEMBL4283577 Ki = 13.8 nM 7.86
CHEMBL494803 Ki = 19.0 nM 7.72
CHEMBL4282476 Ki = 38.0 nM 7.42
CHEMBL4285058 Ki = 104.0 nM 6.98
CHEMBL4286937 Ki = 157.0 nM 6.80
CHEMBL4294163 Ki = 186.0 nM 6.73
CHEMBL4280124 Ki = 254.0 nM 6.59
CHEMBL4290761 Ki = 395.0 nM 6.40
CHEMBL4277116 Ki = 410.0 nM 6.39
CHEMBL515123 IC50 = 630.96 nM 6.20
CHEMBL4283577 IC50 = 794.33 nM 6.10
CHEMBL494803 IC50 = 1000.0 nM 6.00
CHEMBL4282476 IC50 = 1995.26 nM 5.70
CHEMBL4285058 IC50 = 5011.87 nM 5.30
CHEMBL4286937 IC50 = 7943.28 nM 5.10
CHEMBL4294163 IC50 = 10000.0 nM 5.00
CHEMBL4280124 IC50 = 12589.25 nM 4.90
CHEMBL4290761 IC50 = 19952.62 nM 4.70
CHEMBL4277116 IC50 = 19952.62 nM 4.70
CHEMBL4279003 Ki > 1000.0 nM -
CHEMBL4290349 Ki > 1000.0 nM -
CHEMBL4290349 IC50 > 10000.0 nM -
CHEMBL4279003 IC50 > 10000.0 nM -