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Assay Detail

CHEMBL4263212

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NECA from recombinant human adenosine A3 receptor expressed in CHO cell membranes measured after 180 mins
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Studies on enantioselectivity of chiral 4-acetylamino-6-alkyloxy-2-alkylthiopyrimidines acting as antagonists of the human A<sub>3</sub> adenosine receptor.
Cosimelli B, Greco G, Laneri S, Novellino E, Sacchi A, Collina S, Rossi D, Cosconati S, Barresi E, Taliani S, Trincavelli ML, Martini C.
Medchemcomm (2018) 9 : 81 -86
PubMed 30108902 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 7.47 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL431733 NAMODENOSON 3.0 1 9.70
CHEMBL4282074 1 9.30
CHEMBL4277994 1 9.22
CHEMBL4285922 1 8.77
CHEMBL464859 NECA 1 7.12
CHEMBL4285494 1 6.74
CHEMBL4288869 1 6.71
CHEMBL4289256 1 6.36
CHEMBL4292670 1 6.24
CHEMBL4278607 1 6.13
CHEMBL4283891 1 5.86
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL431733 NAMODENOSON Ki = 0.2 nM 9.70
CHEMBL4282074 Ki = 0.5 nM 9.30
CHEMBL4277994 Ki = 0.6 nM 9.22
CHEMBL4285922 Ki = 1.7 nM 8.77
CHEMBL464859 NECA Ki = 75.5 nM 7.12
CHEMBL4285494 Ki = 183.2 nM 6.74
CHEMBL4288869 Ki = 195.1 nM 6.71
CHEMBL4289256 Ki = 440.2 nM 6.36
CHEMBL4292670 Ki = 580.1 nM 6.24
CHEMBL4278607 Ki = 737.5 nM 6.13
CHEMBL4283891 Ki = 1390.0 nM 5.86
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] Ki > 1000.0 nM -