Assay Detail
Binding
CHEMBL4263348
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HIV1 group M subtype B reverse transcriptase p66 A502F mutant RNase H activity using 18-nucleotide 3'-fluorescein-labeled RNA/5'-dabsyl-labeled DNA hybrid as substrate after 1 hr by fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | HIV-1 group M |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247) ↗| Type | SINGLE PROTEIN |
| Organism | Human immunodeficiency virus 1 |
Publication
Structure-guided approach identifies a novel class of HIV-1 ribonuclease H inhibitors: binding mode insights through magnesium complexation and site-directed mutagenesis studies.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.10 | 6.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL550937 | BETA-THUJAPLICINOL | — | 1 | 6.77 |
| CHEMBL4286832 | — | — | 1 | 4.58 |
| CHEMBL4291330 | — | — | 1 | 4.57 |
| CHEMBL4287587 | — | — | 1 | 4.49 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL550937 | BETA-THUJAPLICINOL | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL4286832 | — | IC50 | = | 26350.0 | nM | 4.58 |
| CHEMBL4291330 | — | IC50 | = | 26720.0 | nM | 4.57 |
| CHEMBL4287587 | — | IC50 | = | 32520.0 | nM | 4.49 |