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Assay Detail

CHEMBL4263978

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ALK3 (unknown origin) using Ulight topo IIa (Thr 1342) peptide as substrate preincubated for 10 mins followed by substrate addition and measured after 1 hr by TR-FRET analysis
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bone morphogenetic protein receptor type-1A (CHEMBL5275)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 3-(4-sulfamoylnaphthyl)pyrazolo[1,5-a]pyrimidines as potent and selective ALK2 inhibitors.
Jiang JK, Huang X, Shamim K, Patel PR, Lee A, Wang AQ, Nguyen K, Tawa G, Cuny GD, Yu PB, Zheng W, Xu X, Sanderson P, Huang W.
Bioorg Med Chem Lett (2018) 28 : 3356 -3362
PubMed 30227946 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.68 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4280599 1 8.15
CHEMBL513147 1 7.72
CHEMBL4283587 1 6.35
CHEMBL4281706 1 5.60
CHEMBL4288921 1 5.46
CHEMBL4283638 1 5.19
CHEMBL4278763 1 5.09
CHEMBL4291950 1 5.05
CHEMBL4293917 1 4.89
CHEMBL4285138 1 4.78
CHEMBL4291891 1 4.19
CHEMBL4288494 1 -
CHEMBL4277180 1 -
CHEMBL4292331 1 -
CHEMBL4278242 1 -
CHEMBL4280585 1 -
CHEMBL4290804 1 -
CHEMBL4277239 1 -
CHEMBL4285523 1 -
CHEMBL4278219 1 -
CHEMBL4288905 1 -
CHEMBL4283315 1 -
CHEMBL4281166 1 -
CHEMBL4279135 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4280599 IC50 = 7.0 nM 8.15
CHEMBL513147 IC50 = 19.0 nM 7.72
CHEMBL4283587 IC50 = 444.0 nM 6.35
CHEMBL4281706 IC50 = 2534.0 nM 5.60
CHEMBL4288921 IC50 = 3466.0 nM 5.46
CHEMBL4283638 IC50 = 6439.0 nM 5.19
CHEMBL4278763 IC50 = 8195.0 nM 5.09
CHEMBL4291950 IC50 = 8897.0 nM 5.05
CHEMBL4293917 IC50 = 12830.0 nM 4.89
CHEMBL4285138 IC50 = 16550.0 nM 4.78
CHEMBL4291891 IC50 = 64750.0 nM 4.19
CHEMBL4288494 IC50 > 10000.0 nM -
CHEMBL4277180 IC50 - -
CHEMBL4292331 IC50 > 10000.0 nM -
CHEMBL4278242 IC50 > 10000.0 nM -
CHEMBL4280585 IC50 > 10000.0 nM -
CHEMBL4290804 IC50 > 10000.0 nM -
CHEMBL4277239 IC50 > 10000.0 nM -
CHEMBL4285523 IC50 > 10000.0 nM -
CHEMBL4278219 IC50 > 10000.0 nM -
CHEMBL4288905 IC50 > 10000.0 nM -
CHEMBL4283315 IC50 > 10000.0 nM -
CHEMBL4281166 IC50 > 10000.0 nM -
CHEMBL4279135 IC50 > 10000.0 nM -