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Assay Detail

CHEMBL4267883

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-tagged ALK cytoplasmic domain (1058 to 1620 residues) expressed in baculovirus expression system after 1 hr by mobility shift assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ALK tyrosine kinase receptor (CHEMBL4247)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dual potent ALK and ROS1 inhibitors combating drug-resistant mutants: Synthesis and biological evaluation of aminopyridine-containing diarylaminopyrimidine derivatives.
Guo M, Zuo D, Zhang J, Xing L, Gou W, Jiang F, Jiang N, Zhang D, Zhai X.
Eur J Med Chem (2018) 158 : 322 -333
PubMed 30223120 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.56 5.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4281394 1 5.85
CHEMBL4277936 1 5.68
CHEMBL4284800 1 5.55
CHEMBL2403108 CERITINIB 4.0 1 5.54
CHEMBL4291608 1 5.50
CHEMBL4285785 1 5.41
CHEMBL4279793 1 5.38
CHEMBL601719 CRIZOTINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4281394 IC50 = 1400.0 nM 5.85
CHEMBL4277936 IC50 = 2100.0 nM 5.68
CHEMBL4284800 IC50 = 2800.0 nM 5.55
CHEMBL2403108 CERITINIB IC50 = 2900.0 nM 5.54
CHEMBL4291608 IC50 = 3200.0 nM 5.50
CHEMBL4285785 IC50 = 3900.0 nM 5.41
CHEMBL4279793 IC50 = 4200.0 nM 5.38
CHEMBL601719 CRIZOTINIB IC50 = 32200.0 nM -