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Assay Detail

CHEMBL4268221

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human JAK2 using poly(Glu:Tyr)(4:1) as substrate preincubated for 20 mins followed by [gamma33P]ATP addition measured after 120 mins by ion exchange filter paper method
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of potent dual inhibitors of JAK2 and HDAC based on fusing the pharmacophores of XL019 and vorinostat.
Chu-Farseeva YY, Mustafa N, Poulsen A, Tan EC, Yen JJY, Chng WJ, Dymock BW.
Eur J Med Chem (2018) 158 : 593 -619
PubMed 30243158 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 7.92 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4290831 1 9.05
CHEMBL4281785 1 8.74
CHEMBL4284388 1 8.59
CHEMBL4279986 1 8.54
CHEMBL4290689 1 8.53
CHEMBL4286867 1 8.51
CHEMBL4280540 1 8.47
CHEMBL4294153 1 8.44
CHEMBL4295224 1 8.41
CHEMBL4289006 1 8.37
CHEMBL4292408 1 8.34
CHEMBL4277267 1 8.18
CHEMBL4290297 1 8.10
CHEMBL4282456 1 8.04
CHEMBL2208024 1 7.86
CHEMBL4278591 1 7.77
CHEMBL4285223 1 7.75
CHEMBL2208025 1 7.71
CHEMBL4283921 1 7.64
CHEMBL4280050 1 7.59
CHEMBL2208023 1 7.56
CHEMBL2208028 1 7.54
CHEMBL4290748 1 7.48
CHEMBL4278951 1 7.38
CHEMBL4291781 1 7.34
CHEMBL4282471 1 7.23
CHEMBL4283513 1 7.02
CHEMBL4294092 1 6.96
CHEMBL4282877 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4290831 IC50 = 0.9 nM 9.05
CHEMBL4281785 IC50 = 1.8 nM 8.74
CHEMBL4284388 IC50 = 2.6 nM 8.59
CHEMBL4279986 IC50 = 2.9 nM 8.54
CHEMBL4290689 IC50 = 2.93 nM 8.53
CHEMBL4286867 IC50 = 3.1 nM 8.51
CHEMBL4280540 IC50 = 3.4 nM 8.47
CHEMBL4294153 IC50 = 3.6 nM 8.44
CHEMBL4295224 IC50 = 3.9 nM 8.41
CHEMBL4289006 IC50 = 4.3 nM 8.37
CHEMBL4292408 IC50 = 4.6 nM 8.34
CHEMBL4277267 IC50 = 6.6 nM 8.18
CHEMBL4290297 IC50 = 7.9 nM 8.10
CHEMBL4282456 IC50 = 9.2 nM 8.04
CHEMBL2208024 IC50 = 13.8 nM 7.86
CHEMBL4278591 IC50 = 17.0 nM 7.77
CHEMBL4285223 IC50 = 18.0 nM 7.75
CHEMBL2208025 IC50 = 19.6 nM 7.71
CHEMBL4283921 IC50 = 23.0 nM 7.64
CHEMBL4280050 IC50 = 25.6 nM 7.59
CHEMBL2208023 IC50 = 27.4 nM 7.56
CHEMBL2208028 IC50 = 28.7 nM 7.54
CHEMBL4290748 IC50 = 33.0 nM 7.48
CHEMBL4278951 IC50 = 41.3 nM 7.38
CHEMBL4291781 IC50 = 46.0 nM 7.34
CHEMBL4282471 IC50 = 58.3 nM 7.23
CHEMBL4283513 IC50 = 95.5 nM 7.02
CHEMBL4294092 IC50 = 109.0 nM 6.96
CHEMBL4282877 IC50 = 239.0 nM 6.62