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Assay Detail

CHEMBL4268222

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HDAC1 (unknown origin) using Arg-His-Lys-Lys(Ac) as substrate preincubated with enzyme followed by substrate addition for 2 hrs and measured for 1.5 hrs at 15 mins time interval by fluorescence assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 1 (CHEMBL325)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of potent dual inhibitors of JAK2 and HDAC based on fusing the pharmacophores of XL019 and vorinostat.
Chu-Farseeva YY, Mustafa N, Poulsen A, Tan EC, Yen JJY, Chng WJ, Dymock BW.
Eur J Med Chem (2018) 158 : 593 -619
PubMed 30243158 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.61 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4278951 1 8.42
CHEMBL4282456 1 7.49
CHEMBL4286867 1 7.25
CHEMBL4292408 1 7.11
CHEMBL4294153 1 7.08
CHEMBL4290748 1 7.05
CHEMBL4289006 1 6.86
CHEMBL4283513 1 6.85
CHEMBL4283921 1 6.84
CHEMBL4295224 1 6.81
CHEMBL4277267 1 6.75
CHEMBL4280050 1 6.58
CHEMBL4281785 1 6.54
CHEMBL4294092 1 6.46
CHEMBL4284388 1 6.42
CHEMBL4280540 1 6.20
CHEMBL4290831 1 6.11
CHEMBL4285223 1 6.03
CHEMBL4278591 1 5.98
CHEMBL4291781 1 5.77
CHEMBL4282471 1 5.47
CHEMBL4290689 1 5.34
CHEMBL4282877 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4278951 IC50 = 3.8 nM 8.42
CHEMBL4282456 IC50 = 32.4 nM 7.49
CHEMBL4286867 IC50 = 56.0 nM 7.25
CHEMBL4292408 IC50 = 77.0 nM 7.11
CHEMBL4294153 IC50 = 83.0 nM 7.08
CHEMBL4290748 IC50 = 89.0 nM 7.05
CHEMBL4289006 IC50 = 139.0 nM 6.86
CHEMBL4283513 IC50 = 142.0 nM 6.85
CHEMBL4283921 IC50 = 145.0 nM 6.84
CHEMBL4295224 IC50 = 155.0 nM 6.81
CHEMBL4277267 IC50 = 179.0 nM 6.75
CHEMBL4280050 IC50 = 260.0 nM 6.58
CHEMBL4281785 IC50 = 289.0 nM 6.54
CHEMBL4294092 IC50 = 348.0 nM 6.46
CHEMBL4284388 IC50 = 384.0 nM 6.42
CHEMBL4280540 IC50 = 637.0 nM 6.20
CHEMBL4290831 IC50 = 774.0 nM 6.11
CHEMBL4285223 IC50 = 934.0 nM 6.03
CHEMBL4278591 IC50 = 1043.0 nM 5.98
CHEMBL4291781 IC50 = 1701.0 nM 5.77
CHEMBL4282471 IC50 = 3350.0 nM 5.47
CHEMBL4290689 IC50 = 4581.0 nM 5.34
CHEMBL4282877 IC50 > 10000.0 nM -