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Assay Detail

CHEMBL4269829

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of human full length PPARgamma expressed in HEK293 cells after 18 hrs by luciferase reporter gene based luminescence assay
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Stereoselective Synthesis of the Isomers of Notoincisol A: Assigment of the Absolute Configuration of this Natural Product and Biological Evaluation.
Rycek L, Ticli V, Pyszkowski J, Latkolik S, Liu X, Atanasov AG, Steinacher T, Bauer R, Schuster D, Dirsch VM, Schnürch M, Ernst M, Mihovilovic MD.
J Nat Prod (2018) 81 : 2419 -2428
PubMed 30362739 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 6.46 7.00
Emax 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL595 PIOGLITAZONE 4.0 2 7.00
CHEMBL3358036 2 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL595 PIOGLITAZONE EC50 = 100.0 nM 7.00
CHEMBL3358036 EC50 = 1200.0 nM 5.92
CHEMBL3358036 Emax = 3.38 % -
CHEMBL595 PIOGLITAZONE Emax = 9.15 % -