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Assay Detail

CHEMBL4270047

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of KDM6A (880 to 1401 residues) (unknown origin) using H3 ( 21 to 44 residues) K27me3 as substrate preincubated for 15 min followed by substrate addition measured after 15 mins by FDH-coupled demethylase assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 6A (CHEMBL2069164)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Engineering of Irreversible Inhibitors against Histone Lysine Demethylase KDM5A.
Horton JR, Woodcock CB, Chen Q, Liu X, Zhang X, Shanks J, Rai G, Mott BT, Jansen DJ, Kales SC, Henderson MJ, Cyr M, Pohida K, Hu X, Shah P, Xu X, Jadhav A, Maloney DJ, Hall MD, Simeonov A, Fu H, Vertino PM, Cheng X.
J Med Chem (2018) 61 : 10588 -10601
PubMed 30392349 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.28 4.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4283844 1 4.41
CHEMBL4294426 1 4.26
CHEMBL3188597 1 4.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4283844 IC50 = 39000.0 nM 4.41
CHEMBL4294426 IC50 = 55000.0 nM 4.26
CHEMBL3188597 IC50 = 69000.0 nM 4.16