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Assay Detail

CHEMBL4272250

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal flag-tagged JNK1 (unknown origin) expressed in baculovirus expression system after 60 mins in presence of [gamma-32P]ATP by topcount scintillation method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 8 (CHEMBL2276)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and biological evaluation of novel triaryl pyrazoline derivatives with dioxane moiety for selective BRAFV600E inhibition.
Li HL, Su MM, Xu YJ, Xu C, Yang YS, Zhu HL.
Eur J Med Chem (2018) 155 : 725 -735
PubMed 29940463 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.04 4.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4294787 1 4.05
CHEMBL4290513 1 4.03
CHEMBL4277125 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4294787 IC50 = 88900.0 nM 4.05
CHEMBL4290513 IC50 = 93600.0 nM 4.03
CHEMBL4277125 IC50 > 200000.0 nM -