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Assay Detail

CHEMBL4273596

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mPGES-1 isolated from microsomes of interleukin-1 beta-stimulated human A549 cells using PGH2 as substrate preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E synthase (CHEMBL5658)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a benzenesulfonamide-based dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase that favorably modulates lipid mediator biosynthesis in inflammation.
Cheung SY, Werner M, Esposito L, Troisi F, Cantone V, Liening S, König S, Gerstmeier J, Koeberle A, Bilancia R, Rizza R, Rossi A, Roviezzo F, Temml V, Schuster D, Stuppner H, Schubert-Zsilavecz M, Werz O, Hanke T, Pace S.
Eur J Med Chem (2018) 156 : 815 -830
PubMed 30053720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 5.61 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4286690 1 6.52
CHEMBL93 ZILEUTON 4.0 1 6.22
CHEMBL4287468 1 6.16
CHEMBL4278377 1 6.16
CHEMBL4287827 1 6.00
CHEMBL4278781 1 5.96
CHEMBL4277234 1 5.92
CHEMBL4284489 1 5.80
CHEMBL4292383 1 5.72
CHEMBL4293928 1 5.72
CHEMBL4291396 1 5.62
CHEMBL4285595 1 5.57
CHEMBL4284176 1 5.57
CHEMBL4287883 1 5.54
CHEMBL4277656 1 5.50
CHEMBL4286325 1 5.47
CHEMBL1774311 1 5.43
CHEMBL4288981 1 5.42
CHEMBL4290525 1 5.40
CHEMBL4289742 1 5.33
CHEMBL4289048 1 5.33
CHEMBL4283338 1 5.30
CHEMBL4278826 1 5.28
CHEMBL4290120 1 5.28
CHEMBL4279919 1 5.27
CHEMBL4281072 1 5.21
CHEMBL4293980 1 5.20
CHEMBL4279548 1 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4286690 IC50 = 300.0 nM 6.52
CHEMBL93 ZILEUTON IC50 = 600.0 nM 6.22
CHEMBL4287468 IC50 = 700.0 nM 6.16
CHEMBL4278377 IC50 = 700.0 nM 6.16
CHEMBL4287827 IC50 = 1000.0 nM 6.00
CHEMBL4278781 IC50 = 1100.0 nM 5.96
CHEMBL4277234 IC50 = 1200.0 nM 5.92
CHEMBL4284489 IC50 = 1600.0 nM 5.80
CHEMBL4292383 IC50 = 1900.0 nM 5.72
CHEMBL4293928 IC50 = 1900.0 nM 5.72
CHEMBL4291396 IC50 = 2400.0 nM 5.62
CHEMBL4285595 IC50 = 2700.0 nM 5.57
CHEMBL4284176 IC50 = 2700.0 nM 5.57
CHEMBL4287883 IC50 = 2900.0 nM 5.54
CHEMBL4277656 IC50 = 3200.0 nM 5.50
CHEMBL4286325 IC50 = 3400.0 nM 5.47
CHEMBL1774311 IC50 = 3700.0 nM 5.43
CHEMBL4288981 IC50 = 3800.0 nM 5.42
CHEMBL4290525 IC50 = 4000.0 nM 5.40
CHEMBL4289742 IC50 = 4700.0 nM 5.33
CHEMBL4289048 IC50 = 4700.0 nM 5.33
CHEMBL4283338 IC50 = 5000.0 nM 5.30
CHEMBL4278826 IC50 = 5300.0 nM 5.28
CHEMBL4290120 IC50 = 5200.0 nM 5.28
CHEMBL4279919 IC50 = 5400.0 nM 5.27
CHEMBL4281072 IC50 = 6200.0 nM 5.21
CHEMBL4293980 IC50 = 6300.0 nM 5.20
CHEMBL4279548 IC50 = 6500.0 nM 5.19