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Assay Detail

CHEMBL4275702

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of recombinant human VDR/RXRalpha expressed in HEK293 cells after 24 hrs by Dual-luciferase reporter gene assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Retinoic acid receptor RXR-alpha/Vitamin D3 receptor (CHEMBL3885632)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Sulfonyl-containing phenyl-pyrrolyl pentane analogues: Novel non-secosteroidal vitamin D receptor modulators with favorable physicochemical properties, pharmacokinetic properties and anti-tumor activity.
Kang ZS, Wang C, Han XL, Wang B, Yuan HL, Hou SY, Hao MX, Du JJ, Li YY, Zhou AW, Zhang C.
Eur J Med Chem (2018) 157 : 1174 -1191
PubMed 30193216 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 8.15 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL846 CALCITRIOL 4.0 1 8.80
CHEMBL4288947 1 8.19
CHEMBL2434990 1 8.17
CHEMBL4290851 1 7.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL846 CALCITRIOL EC50 = 1.58 nM 8.80
CHEMBL4288947 EC50 = 6.41 nM 8.19
CHEMBL2434990 EC50 = 6.81 nM 8.17
CHEMBL4290851 EC50 = 38.2 nM 7.42