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Assay Detail

CHEMBL4305804

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length N-terminal GST-tagged CDK4 (1 to 303 residues)/cyclin D3 (1 to 292 residues) expressed in baculovirus expression system using eIF4E-binding protein 1 peptide and ATP as substrate incubated for 30 mins by LANCE ultra kinase assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK4/Cyclin D3 (CHEMBL3038472)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of 6-(2-(dimethylamino)ethyl)-N-(5-fluoro-4-(4-fluoro-1-isopropyl-2-methyl-1H-benzo[d]imidazole-6-yl)pyrimidin-2-yl)-5,6,7,8-tetrahydro-1,6-naphthyridin-2-amine as a highly potent cyclin-dependent kinase 4/6 inhibitor for treatment of cancer.
Shi C, Wang Q, Liao X, Ge H, Huo G, Zhang L, Chen N, Zhai X, Hong Y, Wang L, Han Y, Xiao W, Wang Z, Shi W, Mao Y, Yu J, Xia G, Liu Y.
Eur J Med Chem (2019) 178 : 352 -364
PubMed 31200237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.74 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4448494 1 9.15
CHEMBL4550287 1 9.02
CHEMBL4463172 1 9.00
CHEMBL4438059 1 8.99
CHEMBL4446626 1 8.98
CHEMBL4462493 1 8.97
CHEMBL4439999 1 8.87
CHEMBL4463716 1 8.87
CHEMBL4463874 1 8.82
CHEMBL4461442 1 8.81
CHEMBL4466497 1 8.80
CHEMBL4453095 1 8.79
CHEMBL4449998 1 8.79
CHEMBL3301610 ABEMACICLIB 4.0 1 8.74
CHEMBL4542247 1 8.73
CHEMBL4455210 1 8.71
CHEMBL4438497 1 8.70
CHEMBL4445727 1 8.63
CHEMBL4593340 1 8.51
CHEMBL4458266 1 8.37
CHEMBL4525034 1 8.32
CHEMBL4461579 1 7.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4448494 IC50 = 0.71 nM 9.15
CHEMBL4550287 IC50 = 0.96 nM 9.02
CHEMBL4463172 IC50 = 0.99 nM 9.00
CHEMBL4438059 IC50 = 1.02 nM 8.99
CHEMBL4446626 IC50 = 1.04 nM 8.98
CHEMBL4462493 IC50 = 1.08 nM 8.97
CHEMBL4439999 IC50 = 1.35 nM 8.87
CHEMBL4463716 IC50 = 1.34 nM 8.87
CHEMBL4463874 IC50 = 1.52 nM 8.82
CHEMBL4461442 IC50 = 1.56 nM 8.81
CHEMBL4466497 IC50 = 1.58 nM 8.80
CHEMBL4453095 IC50 = 1.61 nM 8.79
CHEMBL4449998 IC50 = 1.63 nM 8.79
CHEMBL3301610 ABEMACICLIB IC50 = 1.82 nM 8.74
CHEMBL4542247 IC50 = 1.84 nM 8.73
CHEMBL4455210 IC50 = 1.94 nM 8.71
CHEMBL4438497 IC50 = 2.0 nM 8.70
CHEMBL4445727 IC50 = 2.35 nM 8.63
CHEMBL4593340 IC50 = 3.1 nM 8.51
CHEMBL4458266 IC50 = 4.26 nM 8.37
CHEMBL4525034 IC50 = 4.83 nM 8.32
CHEMBL4461579 IC50 = 17.7 nM 7.75