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Assay Detail

CHEMBL4305904

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of pDEST8HisGSTTev-tagged human RIP1 (1 to 375 residues) expressed in baculovirus infected insect cells preincubated with enzyme for 1 hr followed by ATP addition and measured after 5 hrs by ADP-Glo kinase assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-interacting serine/threonine-protein kinase 1 (CHEMBL5464)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a RIP1 Kinase Inhibitor Clinical Candidate (GSK3145095) for the Treatment of Pancreatic Cancer.
Harris PA, Marinis JM, Lich JD, Berger SB, Chirala A, Cox JA, Eidam PM, Finger JN, Gough PJ, Jeong JU, Kang J, Kasparcova V, Leister LK, Mahajan MK, Miller G, Nagilla R, Ouellette MT, Reilly MA, Rendina AR, Rivera EJ, Sun HH, Thorpe JH, Totoritis RD, Wang W, Wu D, Zhang D, Bertin J, Marquis RW.
ACS Med Chem Lett (2019) 10 : 857 -862
PubMed 31223438 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 8.10 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4071864 GSK2982772 2.0 1 9.00
CHEMBL3785703 1 8.80
CHEMBL4554676 1 8.30
CHEMBL4452233 1 8.20
CHEMBL4453758 1 8.20
CHEMBL3786293 1 7.50
CHEMBL4068352 1 7.50
CHEMBL4470945 1 7.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4071864 GSK2982772 IC50 = 1.0 nM 9.00
CHEMBL3785703 IC50 = 1.6 nM 8.80
CHEMBL4554676 IC50 = 5.0 nM 8.30
CHEMBL4452233 IC50 = 6.3 nM 8.20
CHEMBL4453758 IC50 = 6.3 nM 8.20
CHEMBL3786293 IC50 = 32.0 nM 7.50
CHEMBL4068352 IC50 = 32.0 nM 7.50
CHEMBL4470945 IC50 = 50.0 nM 7.30