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Assay Detail

CHEMBL4307109

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of ED-tagged Nrf2 in human U2OS cells co-expressing Keap1 assessed as induction of NRF2 translocation to nucleus after 6 hrs by beta-galactosidase based chemiluminescent assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear factor erythroid 2-related factor 2 (CHEMBL1075094)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Antioxidant, Anti-inflammatory, and Neuroprotective Effects of Novel Vinyl Sulfonate Compounds as Nrf2 Activator.
Choi JW, Shin SJ, Kim HJ, Park JH, Kim HJ, Lee EH, Pae AN, Bahn YS, Park KD.
ACS Med Chem Lett (2019) 10 : 1061 -1067
PubMed 31312409 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 6.12 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4584457 1 7.12
CHEMBL4444710 1 6.78
CHEMBL4468759 1 6.62
CHEMBL48802 SULFORAPHANE 3.0 1 6.24
CHEMBL4526321 1 6.16
CHEMBL4461002 1 6.03
CHEMBL4438169 1 6.02
CHEMBL443343 1 5.98
CHEMBL4563801 1 5.94
CHEMBL4435986 1 5.88
CHEMBL469941 1 5.83
CHEMBL4567717 1 5.75
CHEMBL4446359 1 5.20
CHEMBL4464048 1 -
CHEMBL4545539 1 -
CHEMBL4576084 1 -
CHEMBL463270 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4584457 EC50 = 76.0 nM 7.12
CHEMBL4444710 EC50 = 165.0 nM 6.78
CHEMBL4468759 EC50 = 237.0 nM 6.62
CHEMBL48802 SULFORAPHANE EC50 = 580.0 nM 6.24
CHEMBL4526321 EC50 = 698.0 nM 6.16
CHEMBL4461002 EC50 = 943.0 nM 6.03
CHEMBL4438169 EC50 = 948.0 nM 6.02
CHEMBL443343 EC50 = 1040.0 nM 5.98
CHEMBL4563801 EC50 = 1155.0 nM 5.94
CHEMBL4435986 EC50 = 1334.0 nM 5.88
CHEMBL469941 EC50 = 1480.0 nM 5.83
CHEMBL4567717 EC50 = 1778.0 nM 5.75
CHEMBL4446359 EC50 = 6350.0 nM 5.20
CHEMBL4464048 EC50 > 10000.0 nM -
CHEMBL4545539 EC50 > 10000.0 nM -
CHEMBL4576084 EC50 > 10000.0 nM -
CHEMBL463270 EC50 > 10000.0 nM -