Assay Detail
Binding
CHEMBL4312178
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant SIRT1 using p53 (379 to 382 residues) conjugated to AMC as substrate incubated for 45 mins in presence of NAD+ by fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NAD-dependent protein deacetylase sirtuin-1 (CHEMBL4506) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Sirtuin inhibition and anti-cancer activities of ethyl 2-benzimidazole-5-carboxylate derivatives.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.12 | 6.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL420311 | — | — | 1 | 6.52 |
| CHEMBL4446030 | — | — | 1 | 5.11 |
| CHEMBL4536622 | — | — | 1 | 4.47 |
| CHEMBL595354 | — | — | 1 | 4.38 |
| CHEMBL4520707 | — | — | 1 | - |
| CHEMBL4469996 | — | — | 1 | - |
| CHEMBL4452096 | — | — | 1 | - |
| CHEMBL224864 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL420311 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL4446030 | — | IC50 | = | 7700.0 | nM | 5.11 |
| CHEMBL4536622 | — | IC50 | = | 33600.0 | nM | 4.47 |
| CHEMBL595354 | — | IC50 | = | 42100.0 | nM | 4.38 |
| CHEMBL4520707 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4469996 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4452096 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL224864 | — | IC50 | - | — | - |