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Assay Detail

CHEMBL4312562

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-(3-Chlorophenyl-4,6-t2)-4-nitrobenzo[c][1,2,5]oxadiazol-5-amine binding to his-tagged HIF-2alpha PAS-B domain (unknown origin) measured after 1 hr by scintillation proximity assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Endothelial PAS domain-containing protein 1 (CHEMBL1744522)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-[(1<i>S</i>,2<i>S</i>,3<i>R</i>)-2,3-Difluoro-1-hydroxy-7-methylsulfonylindan-4-yl]oxy-5-fluorobenzonitrile (PT2977), a Hypoxia-Inducible Factor 2α (HIF-2α) Inhibitor for the Treatment of Clear Cell Renal Cell Carcinoma.
Xu R, Wang K, Rizzi JP, Huang H, Grina JA, Schlachter ST, Wang B, Wehn PM, Yang H, Dixon DD, Czerwinski RM, Du X, Ged EL, Han G, Tan H, Wong T, Xie S, Josey JA, Wallace EM.
J Med Chem (2019) 62 : 6876 -6893
PubMed 31282155 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.92 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4585668 BELZUTIFAN 4.0 1 8.05
CHEMBL4453227 1 8.05
CHEMBL4173075 1 7.92
CHEMBL4169671 1 7.39
CHEMBL4437382 1 6.80
CHEMBL4467490 1 6.68
CHEMBL4170809 1 6.46
CHEMBL4516708 1 5.72
CHEMBL4441175 1 5.19
CHEMBL4434904 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4585668 BELZUTIFAN IC50 = 9.0 nM 8.05
CHEMBL4453227 IC50 = 9.0 nM 8.05
CHEMBL4173075 IC50 = 12.0 nM 7.92
CHEMBL4169671 IC50 = 41.0 nM 7.39
CHEMBL4437382 IC50 = 160.0 nM 6.80
CHEMBL4467490 IC50 = 210.0 nM 6.68
CHEMBL4170809 IC50 = 350.0 nM 6.46
CHEMBL4516708 IC50 = 1900.0 nM 5.72
CHEMBL4441175 IC50 = 6500.0 nM 5.19
CHEMBL4434904 IC50 > 10000.0 nM -