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Assay Detail

CHEMBL4312563

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIF-2alpha (unknown origin) expressed in human 786-O cells measured after 24 hrs by one-glo luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Endothelial PAS domain-containing protein 1 (CHEMBL1744522)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-[(1<i>S</i>,2<i>S</i>,3<i>R</i>)-2,3-Difluoro-1-hydroxy-7-methylsulfonylindan-4-yl]oxy-5-fluorobenzonitrile (PT2977), a Hypoxia-Inducible Factor 2α (HIF-2α) Inhibitor for the Treatment of Clear Cell Renal Cell Carcinoma.
Xu R, Wang K, Rizzi JP, Huang H, Grina JA, Schlachter ST, Wang B, Wehn PM, Yang H, Dixon DD, Czerwinski RM, Du X, Ged EL, Han G, Tan H, Wong T, Xie S, Josey JA, Wallace EM.
J Med Chem (2019) 62 : 6876 -6893
PubMed 31282155 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 6.97 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4453227 1 8.40
CHEMBL4585668 BELZUTIFAN 4.0 1 7.96
CHEMBL4173075 1 7.57
CHEMBL4169671 1 7.41
CHEMBL4467490 1 6.34
CHEMBL4170809 1 6.31
CHEMBL4437382 1 6.03
CHEMBL4516708 1 5.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4453227 EC50 = 4.0 nM 8.40
CHEMBL4585668 BELZUTIFAN EC50 = 11.0 nM 7.96
CHEMBL4173075 EC50 = 27.0 nM 7.57
CHEMBL4169671 EC50 = 39.0 nM 7.41
CHEMBL4467490 EC50 = 460.0 nM 6.34
CHEMBL4170809 EC50 = 490.0 nM 6.31
CHEMBL4437382 EC50 = 930.0 nM 6.03
CHEMBL4516708 EC50 = 1900.0 nM 5.72