Assay Detail
Binding
CHEMBL4313181
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human NPP3 expressed in COS-7 cell membranes assessed as reduction in p-nitrophenol production using pNP-TMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and measured after 35 mins
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | COS-7 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (CHEMBL5580) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis, biological evaluation, and docking studies of new raloxifene sulfonate or sulfamate derivatives as inhibitors of nucleotide pyrophosphatase/phosphodiesterase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 6.05 | 6.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4540180 | — | — | 1 | 6.64 |
| CHEMBL4561757 | — | — | 1 | 6.31 |
| CHEMBL4473374 | — | — | 1 | 6.15 |
| CHEMBL4589775 | — | — | 1 | 5.91 |
| CHEMBL4556663 | — | — | 1 | 5.82 |
| CHEMBL4474803 | — | — | 1 | 5.81 |
| CHEMBL4443387 | — | — | 1 | 5.74 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4540180 | — | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL4561757 | — | IC50 | = | 490.0 | nM | 6.31 |
| CHEMBL4473374 | — | IC50 | = | 710.0 | nM | 6.15 |
| CHEMBL4589775 | — | IC50 | = | 1240.0 | nM | 5.91 |
| CHEMBL4556663 | — | IC50 | = | 1510.0 | nM | 5.82 |
| CHEMBL4474803 | — | IC50 | = | 1550.0 | nM | 5.81 |
| CHEMBL4443387 | — | IC50 | = | 1810.0 | nM | 5.74 |