Assay Detail
Binding
CHEMBL4322971
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Trypanosoma cruzi Cruzain expressed in Pichia pastoris using Z-Phe-Arg-AMC substrate incubated for 30 mins by fluorescence based assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Trypanosoma cruzi |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design of Gallinamide A Analogs as Potent Inhibitors of the Cysteine Proteases Human Cathepsin L and <i>Trypanosoma cruzi</i> Cruzain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 16 | 8.83 | 9.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4447348 | — | — | 1 | 9.60 |
| CHEMBL541700 | GALLINAMIDE A | — | 1 | 9.59 |
| CHEMBL4527930 | — | — | 1 | 9.33 |
| CHEMBL4482954 | — | — | 1 | 9.31 |
| CHEMBL4445744 | — | — | 1 | 9.14 |
| CHEMBL4564747 | — | — | 1 | 9.12 |
| CHEMBL4527329 | — | — | 1 | 9.08 |
| CHEMBL4441303 | — | — | 1 | 8.97 |
| CHEMBL4474668 | — | — | 1 | 8.97 |
| CHEMBL4527867 | — | — | 1 | 8.94 |
| CHEMBL4475810 | — | — | 1 | 8.92 |
| CHEMBL4471760 | — | — | 1 | 8.87 |
| CHEMBL4457500 | — | — | 1 | 8.54 |
| CHEMBL4582558 | — | — | 1 | 8.43 |
| CHEMBL4514842 | — | — | 1 | 7.43 |
| CHEMBL4468530 | — | — | 1 | 7.09 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4447348 | — | IC50 | = | 0.25 | nM | 9.60 |
| CHEMBL541700 | GALLINAMIDE A | IC50 | = | 0.26 | nM | 9.59 |
| CHEMBL4527930 | — | IC50 | = | 0.47 | nM | 9.33 |
| CHEMBL4482954 | — | IC50 | = | 0.49 | nM | 9.31 |
| CHEMBL4445744 | — | IC50 | = | 0.73 | nM | 9.14 |
| CHEMBL4564747 | — | IC50 | = | 0.76 | nM | 9.12 |
| CHEMBL4527329 | — | IC50 | = | 0.84 | nM | 9.08 |
| CHEMBL4441303 | — | IC50 | = | 1.08 | nM | 8.97 |
| CHEMBL4474668 | — | IC50 | = | 1.07 | nM | 8.97 |
| CHEMBL4527867 | — | IC50 | = | 1.15 | nM | 8.94 |
| CHEMBL4475810 | — | IC50 | = | 1.2 | nM | 8.92 |
| CHEMBL4471760 | — | IC50 | = | 1.35 | nM | 8.87 |
| CHEMBL4457500 | — | IC50 | = | 2.89 | nM | 8.54 |
| CHEMBL4582558 | — | IC50 | = | 3.69 | nM | 8.43 |
| CHEMBL4514842 | — | IC50 | = | 37.14 | nM | 7.43 |
| CHEMBL4468530 | — | IC50 | = | 81.0 | nM | 7.09 |