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Assay Detail

CHEMBL4325177

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human CDK4 using biotinylated-aminohexyl-Ala-Ala-Ala-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser(PO3)-Tyr-Arg-amide as substrate after 40 mins in presence of [gamma-32P]ATP by scintillation proximity assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 4 (CHEMBL331)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selectivity and Physicochemical Optimization of Repurposed Pyrazolo[1,5-<i>b</i>]pyridazines for the Treatment of Human African Trypanosomiasis.
Tear WF, Bag S, Diaz-Gonzalez R, Ceballos-Pérez G, Rojas-Barros DI, Cordon-Obras C, Pérez-Moreno G, García-Hernández R, Martinez-Martinez MS, Ruiz-Perez LM, Gamarro F, Gonzalez Pacanowska D, Caffrey CR, Ferrins L, Manzano P, Navarro M, Pollastri MP.
J Med Chem (2020) 63 : 756 -783
PubMed 31846577 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.62 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL359999 GW801372X 1 7.70
CHEMBL187081 PYRAZOLOPYRIDAZINE 1 1 7.20
CHEMBL359554 1 6.80
CHEMBL361894 GW806290X 1 6.50
CHEMBL186101 1 5.80
CHEMBL364587 1 5.70
CHEMBL365248 GW810372X 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL359999 GW801372X IC50 = 19.95 nM 7.70
CHEMBL187081 PYRAZOLOPYRIDAZINE 1 IC50 = 63.1 nM 7.20
CHEMBL359554 IC50 = 158.49 nM 6.80
CHEMBL361894 GW806290X IC50 = 316.23 nM 6.50
CHEMBL186101 IC50 = 1584.89 nM 5.80
CHEMBL364587 IC50 = 1995.26 nM 5.70
CHEMBL365248 GW810372X IC50 > 19952.62 nM -