Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4325706

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human Myc-DDK-tagged IDH1 R132H mutant using alpha-ketoglutarate as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 45 mins by diaphorase/resazurin dye based assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design and Identification of FT-2102 (Olutasidenib), a Potent Mutant-Selective IDH1 Inhibitor.
Caravella JA, Lin J, Diebold RB, Campbell AM, Ericsson A, Gustafson G, Wang Z, Castro J, Clarke A, Gotur D, Josephine HR, Katz M, Kershaw M, Yao L, Toms AV, Barr KJ, Dinsmore CJ, Walker D, Ashwell S, Lu W.
J Med Chem (2020) 63 : 1612 -1623
PubMed 31971798 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.13 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4456610 1 8.10
CHEMBL4469055 1 8.10
CHEMBL4437754 1 7.96
CHEMBL4464313 1 7.96
CHEMBL4297610 OLUTASIDENIB 4.0 1 7.67
CHEMBL4446657 1 7.60
CHEMBL4547257 1 7.39
CHEMBL4454572 1 7.19
CHEMBL4461178 1 6.80
CHEMBL4547484 1 6.65
CHEMBL4446598 1 6.45
CHEMBL4456162 1 6.16
CHEMBL4533443 1 4.60
CHEMBL4532955 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4456610 IC50 = 8.0 nM 8.10
CHEMBL4469055 IC50 = 8.0 nM 8.10
CHEMBL4437754 IC50 = 11.0 nM 7.96
CHEMBL4464313 IC50 = 11.0 nM 7.96
CHEMBL4297610 OLUTASIDENIB IC50 = 21.2 nM 7.67
CHEMBL4446657 IC50 = 25.0 nM 7.60
CHEMBL4547257 IC50 = 40.3 nM 7.39
CHEMBL4454572 IC50 = 64.7 nM 7.19
CHEMBL4461178 IC50 = 160.0 nM 6.80
CHEMBL4547484 IC50 = 223.0 nM 6.65
CHEMBL4446598 IC50 = 358.0 nM 6.45
CHEMBL4456162 IC50 = 694.0 nM 6.16
CHEMBL4533443 IC50 = 25000.0 nM 4.60
CHEMBL4532955 IC50 > 25000.0 nM -