Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4327486

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MDR1 (unknown origin) expressed in MDCK cells assessed as reduction in calcein-AM efflux preincubated for 30 mins followed by calcein-AM addition measured after 30 mins by spectrofluorimetric method
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,2,3,4-Tetrahydroisoquinoline/2H-chromen-2-one conjugates as nanomolar P-glycoprotein inhibitors: Molecular determinants for affinity and selectivity over multidrug resistance associated protein 1.
Rullo M, Niso M, Pisani L, Carrieri A, Colabufo NA, Cellamare S, Altomare CD.
Eur J Med Chem (2019) 161 : 433 -444
PubMed 30384046 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.35 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4592250 1 7.16
CHEMBL4476238 1 6.82
CHEMBL4539336 1 6.80
CHEMBL4473596 1 6.80
CHEMBL4462737 1 6.72
CHEMBL4450854 1 6.72
CHEMBL4569990 1 6.72
CHEMBL4571279 1 6.66
CHEMBL4464239 1 6.66
CHEMBL4474108 1 6.55
CHEMBL4452234 1 6.48
CHEMBL4436042 1 6.42
CHEMBL4563027 1 6.41
CHEMBL4451488 1 6.39
CHEMBL4466035 1 6.34
CHEMBL4514827 1 6.26
CHEMBL4562150 1 6.25
CHEMBL4541580 1 6.11
CHEMBL4474386 1 6.04
CHEMBL4553878 1 6.00
CHEMBL260599 1 5.92
CHEMBL6966 VERAPAMIL 4.0 1 5.89
CHEMBL4574355 1 5.57
CHEMBL4435844 1 4.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4592250 IC50 = 70.0 nM 7.16
CHEMBL4476238 IC50 = 150.0 nM 6.82
CHEMBL4539336 IC50 = 160.0 nM 6.80
CHEMBL4473596 IC50 = 160.0 nM 6.80
CHEMBL4462737 IC50 = 190.0 nM 6.72
CHEMBL4450854 IC50 = 190.0 nM 6.72
CHEMBL4569990 IC50 = 190.0 nM 6.72
CHEMBL4571279 IC50 = 220.0 nM 6.66
CHEMBL4464239 IC50 = 220.0 nM 6.66
CHEMBL4474108 IC50 = 280.0 nM 6.55
CHEMBL4452234 IC50 = 330.0 nM 6.48
CHEMBL4436042 IC50 = 380.0 nM 6.42
CHEMBL4563027 IC50 = 390.0 nM 6.41
CHEMBL4451488 IC50 = 410.0 nM 6.39
CHEMBL4466035 IC50 = 460.0 nM 6.34
CHEMBL4514827 IC50 = 550.0 nM 6.26
CHEMBL4562150 IC50 = 560.0 nM 6.25
CHEMBL4541580 IC50 = 770.0 nM 6.11
CHEMBL4474386 IC50 = 910.0 nM 6.04
CHEMBL4553878 IC50 = 1000.0 nM 6.00
CHEMBL260599 IC50 = 1200.0 nM 5.92
CHEMBL6966 VERAPAMIL IC50 = 1300.0 nM 5.89
CHEMBL4574355 IC50 = 2700.0 nM 5.57
CHEMBL4435844 IC50 = 15000.0 nM 4.82