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Assay Detail

CHEMBL4328827

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCB1 in human A2780/ADR cells assessed as calcein-AM accumulation preincubated for 30 mins followed by calcein-AM addition measured over 60 mins at 60 secs time interval by fluorescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of quinazoline derivatives - A SAR study of novel inhibitors of ABCG2.
Krapf MK, Gallus J, Spindler A, Wiese M.
Eur J Med Chem (2019) 161 : 506 -525
PubMed 30390439 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.34 5.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL160 CYCLOSPORINE 4.0 1 5.93
CHEMBL4475108 1 5.74
CHEMBL4446794 1 5.73
CHEMBL4448170 1 5.09
CHEMBL1307612 1 4.83
CHEMBL4469532 1 4.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL160 CYCLOSPORINE IC50 = 1170.0 nM 5.93
CHEMBL4475108 IC50 = 1810.0 nM 5.74
CHEMBL4446794 IC50 = 1880.0 nM 5.73
CHEMBL4448170 IC50 = 8030.0 nM 5.09
CHEMBL1307612 IC50 = 14700.0 nM 4.83
CHEMBL4469532 IC50 = 18800.0 nM 4.73