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Assay Detail

CHEMBL4328892

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human COX-2 expressed in Baculovirus infected sf9 cells using arachidonic acid as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by ELISA
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 2 (CHEMBL230)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Medicinal chemistry of vicinal diaryl scaffold: A mini review.
Ramajayam R.
Eur J Med Chem (2019) 162 : 1 -17
PubMed 30396033 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.33 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL52760 1 8.70
CHEMBL865 VALDECOXIB 4.0 1 8.30
CHEMBL92154 1 8.12
CHEMBL4533150 1 7.96
CHEMBL4473838 1 7.70
CHEMBL274893 SC-57666 1 7.58
CHEMBL89429 1 7.58
CHEMBL118 CELECOXIB 4.0 1 7.40
CHEMBL4527106 1 7.22
CHEMBL328837 1 7.10
CHEMBL109017 1 6.96
CHEMBL325755 1 5.73
CHEMBL43511 1 4.99
CHEMBL55189 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL52760 IC50 = 2.0 nM 8.70
CHEMBL865 VALDECOXIB IC50 = 5.0 nM 8.30
CHEMBL92154 IC50 = 7.5 nM 8.12
CHEMBL4533150 IC50 = 11.0 nM 7.96
CHEMBL4473838 IC50 = 20.0 nM 7.70
CHEMBL274893 SC-57666 IC50 = 26.0 nM 7.58
CHEMBL89429 IC50 = 26.0 nM 7.58
CHEMBL118 CELECOXIB IC50 = 40.0 nM 7.40
CHEMBL4527106 IC50 = 60.0 nM 7.22
CHEMBL328837 IC50 = 80.0 nM 7.10
CHEMBL109017 IC50 = 110.0 nM 6.96
CHEMBL325755 IC50 = 1850.0 nM 5.73
CHEMBL43511 IC50 = 10200.0 nM 4.99
CHEMBL55189 IC50 > 100000.0 nM -