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Assay Detail

CHEMBL4329571

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

80S Ribosome (CHEMBL3987582)
Type PROTEIN NUCLEIC-ACID COMPLEX
Organism Homo sapiens

Publication

Discovery of N-(piperidin-3-yl)-N-(pyridin-2-yl)piperidine/piperazine-1-carboxamides as small molecule inhibitors of PCSK9.
Londregan AT, Aspnes G, Limberakis C, Loria PM, McClure KF, Petersen DN, Raymer B, Ruggeri RB, Wei L, Xiao J, Piotrowski DW.
Bioorg Med Chem Lett (2018) 28 : 3685 -3688
PubMed 30482620 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.73 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4574496 1 6.30
CHEMBL4560206 1 6.30
CHEMBL4554909 1 6.04
CHEMBL4534859 1 5.81
CHEMBL4446635 1 5.68
CHEMBL4533299 1 5.64
CHEMBL4566239 1 5.63
CHEMBL4469712 1 5.44
CHEMBL4527910 1 5.36
CHEMBL4128250 1 5.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4574496 IC50 = 500.0 nM 6.30
CHEMBL4560206 IC50 = 500.0 nM 6.30
CHEMBL4554909 IC50 = 920.0 nM 6.04
CHEMBL4534859 IC50 = 1540.0 nM 5.81
CHEMBL4446635 IC50 = 2080.0 nM 5.68
CHEMBL4533299 IC50 = 2270.0 nM 5.64
CHEMBL4566239 IC50 = 2330.0 nM 5.63
CHEMBL4469712 IC50 = 3630.0 nM 5.44
CHEMBL4527910 IC50 = 4380.0 nM 5.36
CHEMBL4128250 IC50 = 8040.0 nM 5.09