Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4329997

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His6-tagged HexB expressed in Pichia pastoris using varying levels of 4-Mu-GlcNAc as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by dixon plot analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-hexosaminidase subunit beta (CHEMBL5877)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Modification of the Thioglycosyl-Naphthalimides as Potent and Selective Human O-GlcNAcase Inhibitors.
Shen S, Dong L, Chen W, Zeng X, Lu H, Yang Q, Zhang J.
ACS Med Chem Lett (2018) 9 : 1241 -1246
PubMed 30613333 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 5.34 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4448965 1 5.92
CHEMBL4452208 1 5.80
CHEMBL4435797 1 5.80
CHEMBL4454357 1 5.75
CHEMBL4548064 1 5.75
CHEMBL4548738 1 5.58
CHEMBL4555514 1 5.36
CHEMBL4540913 1 5.29
CHEMBL4438655 1 4.66
CHEMBL4474777 1 4.61
CHEMBL4559735 1 4.25
CHEMBL4564023 1 -
CHEMBL4440132 1 -
CHEMBL4455894 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4448965 Ki = 1200.0 nM 5.92
CHEMBL4452208 Ki = 1600.0 nM 5.80
CHEMBL4435797 Ki = 1600.0 nM 5.80
CHEMBL4454357 Ki = 1800.0 nM 5.75
CHEMBL4548064 Ki = 1800.0 nM 5.75
CHEMBL4548738 Ki = 2600.0 nM 5.58
CHEMBL4555514 Ki = 4400.0 nM 5.36
CHEMBL4540913 Ki = 5100.0 nM 5.29
CHEMBL4438655 Ki = 22100.0 nM 4.66
CHEMBL4474777 Ki = 24700.0 nM 4.61
CHEMBL4559735 Ki = 56200.0 nM 4.25
CHEMBL4564023 Ki - -
CHEMBL4440132 Ki - -
CHEMBL4455894 Ki - -