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Assay Detail

CHEMBL4331432

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GST-tagged BRAF V600E mutant (416 to 766 residues) using human full length 6His-tagged MEK1 (K97R) as substrate preincubated for 20 mins followed by [gamma-33P]-ATP addition and measured after 120 mins by filter binding method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase B-raf (CHEMBL5145)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent Pan-Raf inhibitors with increased solubility to overcome drug resistance.
Wang L, Zhang Y, Zhang Q, Zhu G, Zhang Z, Duan C, Lu T, Tang W.
Eur J Med Chem (2019) 163 : 243 -255
PubMed 30529543 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.08 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4438001 1 9.70
CHEMBL4545914 1 8.92
CHEMBL4559599 1 8.68
CHEMBL4518581 1 8.64
CHEMBL4438236 1 8.46
CHEMBL3577124 LY-3009120 1.0 1 8.26
CHEMBL4457131 1 8.21
CHEMBL4439157 1 8.13
CHEMBL4471370 1 7.91
CHEMBL4555839 1 7.87
CHEMBL4436132 1 7.87
CHEMBL4586402 1 7.80
CHEMBL4588013 1 7.77
CHEMBL1229517 VEMURAFENIB 4.0 1 7.50
CHEMBL4538140 1 7.47
CHEMBL4473290 1 7.46
CHEMBL1336 SORAFENIB 4.0 1 7.42
CHEMBL4518466 1 7.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4438001 IC50 = 0.2 nM 9.70
CHEMBL4545914 IC50 = 1.2 nM 8.92
CHEMBL4559599 IC50 = 2.1 nM 8.68
CHEMBL4518581 IC50 = 2.3 nM 8.64
CHEMBL4438236 IC50 = 3.5 nM 8.46
CHEMBL3577124 LY-3009120 IC50 = 5.5 nM 8.26
CHEMBL4457131 IC50 = 6.2 nM 8.21
CHEMBL4439157 IC50 = 7.4 nM 8.13
CHEMBL4471370 IC50 = 12.3 nM 7.91
CHEMBL4555839 IC50 = 13.4 nM 7.87
CHEMBL4436132 IC50 = 13.4 nM 7.87
CHEMBL4586402 IC50 = 15.8 nM 7.80
CHEMBL4588013 IC50 = 17.0 nM 7.77
CHEMBL1229517 VEMURAFENIB IC50 = 31.6 nM 7.50
CHEMBL4538140 IC50 = 34.1 nM 7.47
CHEMBL4473290 IC50 = 34.5 nM 7.46
CHEMBL1336 SORAFENIB IC50 = 38.1 nM 7.42
CHEMBL4518466 IC50 = 43.7 nM 7.36