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Assay Detail

CHEMBL4331651

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Plasmodium falciparum plasmepsin 1 using DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS as substrate preincubated for 30 mins followed by substrate addition and measured every min for 8 to 15 mins by FRET assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Plasmepsin I (CHEMBL4687)
Type SINGLE PROTEIN
Organism Plasmodium falciparum

Publication

Peptidomimetic plasmepsin inhibitors with potent anti-malarial activity and selectivity against cathepsin D.
Zogota R, Kinena L, Withers-Martinez C, Blackman MJ, Bobrovs R, Pantelejevs T, Kanepe-Lapsa I, Ozola V, Jaudzems K, Suna E, Jirgensons A.
Eur J Med Chem (2019) 163 : 344 -352
PubMed 30529637 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.61 6.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4449824 1 6.11
CHEMBL4475791 1 6.10
CHEMBL4449532 1 5.96
CHEMBL4555241 1 5.75
CHEMBL4515147 1 5.70
CHEMBL4454293 1 5.60
CHEMBL2334376 1 5.51
CHEMBL4464784 1 5.25
CHEMBL4515257 1 5.13
CHEMBL4520253 1 4.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4449824 IC50 = 780.0 nM 6.11
CHEMBL4475791 IC50 = 800.0 nM 6.10
CHEMBL4449532 IC50 = 1100.0 nM 5.96
CHEMBL4555241 IC50 = 1800.0 nM 5.75
CHEMBL4515147 IC50 = 2000.0 nM 5.70
CHEMBL4454293 IC50 = 2500.0 nM 5.60
CHEMBL2334376 IC50 = 3100.0 nM 5.51
CHEMBL4464784 IC50 = 5600.0 nM 5.25
CHEMBL4515257 IC50 = 7400.0 nM 5.13
CHEMBL4520253 IC50 = 10300.0 nM 4.99