Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4332269

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-fused IDH1 R132H mutant expressed in Escherichia coli using alpha-ketoglutarate as substrate preincubated for 15 mins followed by substrate addition
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of 3-aryl-4-indolyl-maleimides as potent mutant isocitrate dehydrogenase-1 inhibitors.
Liu X, Hu Y, Gao A, Xu M, Gao L, Xu L, Zhou Y, Gao J, Ye Q, Li J.
Bioorg Med Chem (2019) 27 : 589 -603
PubMed 30600148 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.06 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2180727 1 7.70
CHEMBL4559482 1 7.30
CHEMBL4476190 1 7.22
CHEMBL4556914 1 7.22
CHEMBL4465287 1 7.05
CHEMBL4469450 1 7.00
CHEMBL4514241 1 6.85
CHEMBL4467855 1 6.72
CHEMBL4544382 1 6.64
CHEMBL4457293 1 6.54
CHEMBL4471506 1 6.09
CHEMBL4524313 1 6.03
CHEMBL4568252 1 6.03
CHEMBL4557985 1 6.02
CHEMBL550052 1 5.89
CHEMBL4454383 1 5.85
CHEMBL4463255 1 5.84
CHEMBL4473155 1 5.83
CHEMBL4548634 1 5.81
CHEMBL4560109 1 5.80
CHEMBL4442650 1 5.70
CHEMBL4459022 1 5.65
CHEMBL4471075 1 5.52
CHEMBL4534551 1 5.43
CHEMBL4446593 1 5.36
CHEMBL4452734 1 5.30
CHEMBL4467139 1 5.10
CHEMBL4453488 1 4.93
CHEMBL4466687 1 4.87
CHEMBL4576887 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2180727 IC50 = 20.0 nM 7.70
CHEMBL4559482 IC50 = 50.0 nM 7.30
CHEMBL4476190 IC50 = 60.0 nM 7.22
CHEMBL4556914 IC50 = 60.0 nM 7.22
CHEMBL4465287 IC50 = 90.0 nM 7.05
CHEMBL4469450 IC50 = 100.0 nM 7.00
CHEMBL4514241 IC50 = 140.0 nM 6.85
CHEMBL4467855 IC50 = 190.0 nM 6.72
CHEMBL4544382 IC50 = 230.0 nM 6.64
CHEMBL4457293 IC50 = 290.0 nM 6.54
CHEMBL4471506 IC50 = 810.0 nM 6.09
CHEMBL4524313 IC50 = 930.0 nM 6.03
CHEMBL4568252 IC50 = 930.0 nM 6.03
CHEMBL4557985 IC50 = 950.0 nM 6.02
CHEMBL550052 IC50 = 1280.0 nM 5.89
CHEMBL4454383 IC50 = 1400.0 nM 5.85
CHEMBL4463255 IC50 = 1450.0 nM 5.84
CHEMBL4473155 IC50 = 1480.0 nM 5.83
CHEMBL4548634 IC50 = 1540.0 nM 5.81
CHEMBL4560109 IC50 = 1580.0 nM 5.80
CHEMBL4442650 IC50 = 1980.0 nM 5.70
CHEMBL4459022 IC50 = 2220.0 nM 5.65
CHEMBL4471075 IC50 = 3050.0 nM 5.52
CHEMBL4534551 IC50 = 3720.0 nM 5.43
CHEMBL4446593 IC50 = 4420.0 nM 5.36
CHEMBL4452734 IC50 = 5000.0 nM 5.30
CHEMBL4467139 IC50 = 7910.0 nM 5.10
CHEMBL4453488 IC50 = 11860.0 nM 4.93
CHEMBL4466687 IC50 = 13620.0 nM 4.87
CHEMBL4576887 IC50 = 39600.0 nM 4.40