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Assay Detail

CHEMBL4333175

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Functional
Agonist activity at NPFF2 receptor (unknown origin) expressed in HEK293A cells assessed as inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins
10
Total Activities
3
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuropeptide FF receptor 2 (CHEMBL5952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of two novel branched peptidomimetics containing endomorphin-2 and RF9 pharmacophores: Synthesis and neuropharmacological evaluation.
Zhang T, Han Z, Shi X, Zhao W, Wang Z, Zhang R, Xu B, Zhang M, Zhang Q, Xiao J, Zhu H, Zheng T, Fang Q.
Bioorg Med Chem (2019) 27 : 630 -643
PubMed 30626554 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.83 7.40
Emax 3 - -
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL429731 2 7.40
CHEMBL4449491 4 6.80
CHEMBL4442534 4 6.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL429731 EC50 = 39.81 nM 7.40
CHEMBL4449491 EC50 = 158.49 nM 6.80
CHEMBL4449491 EC50 = 170.0 nM 6.77
CHEMBL4442534 EC50 = 251.19 nM 6.60
CHEMBL4442534 EC50 = 250.0 nM 6.60
CHEMBL4442534 Emax = 31.28 % -
CHEMBL4442534 Inhibition - % -
CHEMBL4449491 Emax = 26.56 % -
CHEMBL4449491 Inhibition - % -
CHEMBL429731 Emax = 52.2 % -