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Assay Detail

CHEMBL4335416

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal 6-His tagged recombinant human DHODH N-terminal domain delta29 mutant expressed in Escherichia coli BL21(DE3) using dihydroorotate as substrate measured every 30 secs for 6 mins by DCIP dye based assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery, Optimization, and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors.
Yang Y, Cao L, Gao H, Wu Y, Wang Y, Fang F, Lan T, Lou Z, Rao Y.
J Med Chem (2019) 62 : 4056 -4073
PubMed 30938999 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.59 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4454780 1 7.22
CHEMBL4558413 1 6.60
CHEMBL973 TERIFLUNOMIDE 4.0 1 6.55
CHEMBL4461601 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4454780 IC50 = 60.0 nM 7.22
CHEMBL4558413 IC50 = 250.0 nM 6.60
CHEMBL973 TERIFLUNOMIDE IC50 = 280.0 nM 6.55
CHEMBL4461601 IC50 = 1030.0 nM 5.99