Assay Detail
Binding
CHEMBL4337389
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
[<sup>3</sup>H]UR-DEBa176: A 2,4-Diaminopyrimidine-Type Radioligand Enabling Binding Studies at the Human, Mouse, and Rat Histamine H<sub>4</sub> Receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 12 | 7.08 | 8.29 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4454158 | — | — | 1 | 8.29 |
| CHEMBL4455324 | — | — | 1 | 8.07 |
| CHEMBL4439142 | — | — | 1 | 7.93 |
| CHEMBL4543119 | — | — | 1 | 7.20 |
| CHEMBL4445423 | — | — | 1 | 7.16 |
| CHEMBL4534254 | — | — | 1 | 6.94 |
| CHEMBL4513989 | — | — | 1 | 6.73 |
| CHEMBL4586599 | — | — | 1 | 6.69 |
| CHEMBL4576393 | — | — | 1 | 6.21 |
| CHEMBL4471442 | — | — | 1 | 5.57 |
| CHEMBL4563834 | — | — | 1 | - |
| CHEMBL4466349 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4454158 | — | Ki | = | 5.129 | nM | 8.29 |
| CHEMBL4455324 | — | Ki | = | 8.511 | nM | 8.07 |
| CHEMBL4439142 | — | Ki | = | 11.75 | nM | 7.93 |
| CHEMBL4543119 | — | Ki | = | 63.1 | nM | 7.20 |
| CHEMBL4445423 | — | Ki | = | 69.18 | nM | 7.16 |
| CHEMBL4534254 | — | Ki | = | 114.82 | nM | 6.94 |
| CHEMBL4513989 | — | Ki | = | 186.21 | nM | 6.73 |
| CHEMBL4586599 | — | Ki | = | 204.17 | nM | 6.69 |
| CHEMBL4576393 | — | Ki | = | 616.6 | nM | 6.21 |
| CHEMBL4471442 | — | Ki | = | 2691.53 | nM | 5.57 |
| CHEMBL4563834 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4466349 | — | Ki | > | 10000.0 | nM | - |