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Assay Detail

CHEMBL4338382

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Trypanosoma brucei rhodesiense rhodesain expressed in Pichia pastoris assessed as inhibition constant measured for 30 mins using Cbz-Phe-Arg-AMC as substrate by fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei rhodesiense
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Rhodesain (CHEMBL4188)
Type SINGLE PROTEIN
Organism Trypanosoma brucei rhodesiense

Publication

Optimization Strategy of Novel Peptide-Based Michael Acceptors for the Treatment of Human African Trypanosomiasis.
Ettari R, Previti S, Maiorana S, Amendola G, Wagner A, Cosconati S, Schirmeister T, Hellmich UA, Zappalà M.
J Med Chem (2019) 62 : 10617 -10629
PubMed 31714776 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 9.51 10.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4436812 1 10.82
CHEMBL4453415 1 10.64
CHEMBL4584291 1 10.54
CHEMBL4437914 1 10.47
CHEMBL4459413 1 9.30
CHEMBL4456010 1 9.13
CHEMBL4456727 1 8.74
CHEMBL4454883 1 8.68
CHEMBL4464737 1 8.51
CHEMBL4438444 1 8.26
CHEMBL4515298 1 -
CHEMBL4475066 1 -
CHEMBL4587965 1 -
CHEMBL4566436 1 -
CHEMBL4441635 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4436812 Ki = 0.015 nM 10.82
CHEMBL4453415 Ki = 0.023 nM 10.64
CHEMBL4584291 Ki = 0.029 nM 10.54
CHEMBL4437914 Ki = 0.034 nM 10.47
CHEMBL4459413 Ki = 0.5 nM 9.30
CHEMBL4456010 Ki = 0.74 nM 9.13
CHEMBL4456727 Ki = 1.8 nM 8.74
CHEMBL4454883 Ki = 2.1 nM 8.68
CHEMBL4464737 Ki = 3.1 nM 8.51
CHEMBL4438444 Ki = 5.54 nM 8.26
CHEMBL4515298 Ki = 0.003 nM -
CHEMBL4475066 Ki = 0.0011 nM -
CHEMBL4587965 Ki = 0.0044 nM -
CHEMBL4566436 Ki = 0.0006 nM -
CHEMBL4441635 Ki = 0.0076 nM -