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Assay Detail

CHEMBL4338879

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length C-terminal His8 tagged IDH1 R132H mutant (1 to 414 residues) (unknown origin) expressed in Escherichia coli BE3 cells/wild type IDH1 (unknown origin) heterodimer assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate after 60 mins by Diaphorase/Resazurin dye based fluorescence assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Vorasidenib (AG-881): A First-in-Class, Brain-Penetrant Dual Inhibitor of Mutant IDH1 and 2 for Treatment of Glioma.
Konteatis Z, Artin E, Nicolay B, Straley K, Padyana AK, Jin L, Chen Y, Narayaraswamy R, Tong S, Wang F, Zhou D, Cui D, Cai Z, Luo Z, Fang C, Tang H, Lv X, Nagaraja R, Yang H, Su SM, Sui Z, Dang L, Yen K, Popovici-Muller J, Codega P, Campos C, Mellinghoff IK, Biller SA.
ACS Med Chem Lett (2020) 11 : 101 -107
PubMed 32071674 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.34 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4278845 1 9.22
CHEMBL4278828 1 8.22
CHEMBL4282989 1 8.22
CHEMBL4280132 1 8.22
CHEMBL4277352 1 8.22
CHEMBL4283785 1 8.15
CHEMBL4280772 1 8.05
CHEMBL4278420 1 8.05
CHEMBL4448857 1 7.70
CHEMBL4289692 1 7.66
CHEMBL4288840 1 7.57
CHEMBL4284907 1 7.22
CHEMBL4292293 1 7.08
CHEMBL4292856 1 6.85
CHEMBL4285495 1 6.73
CHEMBL4276904 1 6.70
CHEMBL4293274 1 6.41
CHEMBL4289685 1 6.17
CHEMBL3989908 ENASIDENIB 4.0 1 6.17
CHEMBL4284596 1 5.80
CHEMBL4277032 1 5.76
CHEMBL4473884 1 -
CHEMBL4560615 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4278845 IC50 = 0.6 nM 9.22
CHEMBL4278828 IC50 = 6.0 nM 8.22
CHEMBL4282989 IC50 = 6.0 nM 8.22
CHEMBL4280132 IC50 = 6.0 nM 8.22
CHEMBL4277352 IC50 = 6.0 nM 8.22
CHEMBL4283785 IC50 = 7.0 nM 8.15
CHEMBL4280772 IC50 = 9.0 nM 8.05
CHEMBL4278420 IC50 = 9.0 nM 8.05
CHEMBL4448857 IC50 = 20.0 nM 7.70
CHEMBL4289692 IC50 = 22.0 nM 7.66
CHEMBL4288840 IC50 = 27.0 nM 7.57
CHEMBL4284907 IC50 = 60.0 nM 7.22
CHEMBL4292293 IC50 = 83.0 nM 7.08
CHEMBL4292856 IC50 = 140.0 nM 6.85
CHEMBL4285495 IC50 = 185.0 nM 6.73
CHEMBL4276904 IC50 = 201.0 nM 6.70
CHEMBL4293274 IC50 = 394.0 nM 6.41
CHEMBL4289685 IC50 = 677.0 nM 6.17
CHEMBL3989908 ENASIDENIB IC50 = 677.0 nM 6.17
CHEMBL4284596 IC50 = 1566.0 nM 5.80
CHEMBL4277032 IC50 = 1749.0 nM 5.76
CHEMBL4473884 IC50 - -
CHEMBL4560615 IC50 - -