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Assay Detail

CHEMBL4338885

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDH1 R132H mutant in glioma patient-derived human TS603 neurosphere cells assessed as reduction in 2-HG content using alpha-ketoglutarate as substrate after 48 hrs by LC-MS analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Vorasidenib (AG-881): A First-in-Class, Brain-Penetrant Dual Inhibitor of Mutant IDH1 and 2 for Treatment of Glioma.
Konteatis Z, Artin E, Nicolay B, Straley K, Padyana AK, Jin L, Chen Y, Narayaraswamy R, Tong S, Wang F, Zhou D, Cui D, Cai Z, Luo Z, Fang C, Tang H, Lv X, Nagaraja R, Yang H, Su SM, Sui Z, Dang L, Yen K, Popovici-Muller J, Codega P, Campos C, Mellinghoff IK, Biller SA.
ACS Med Chem Lett (2020) 11 : 101 -107
PubMed 32071674 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 8.88 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4278845 1 9.60
CHEMBL4283785 1 9.52
CHEMBL4280132 1 9.15
CHEMBL4280772 1 9.00
CHEMBL4277352 1 9.00
CHEMBL4278828 1 8.70
CHEMBL4288840 1 8.40
CHEMBL4282989 1 8.40
CHEMBL4289692 1 8.15
CHEMBL4292293 1 -
CHEMBL4284907 1 -
CHEMBL4278420 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4278845 IC50 = 0.25 nM 9.60
CHEMBL4283785 IC50 = 0.3 nM 9.52
CHEMBL4280132 IC50 = 0.7 nM 9.15
CHEMBL4280772 IC50 = 1.0 nM 9.00
CHEMBL4277352 IC50 = 1.0 nM 9.00
CHEMBL4278828 IC50 = 2.0 nM 8.70
CHEMBL4288840 IC50 = 4.0 nM 8.40
CHEMBL4282989 IC50 = 4.0 nM 8.40
CHEMBL4289692 IC50 = 7.0 nM 8.15
CHEMBL4292293 IC50 - -
CHEMBL4284907 IC50 - -
CHEMBL4278420 IC50 - -